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KMID : 0370220230670060335
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Yakhak Hoeji
2023 Volume.67 No. 6 p.335 ~ p.341
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Effect of Oleanolic Acid on the Gene Expression of Pulmonary MUC5AC Mucin through IKK/I?B?/NF-?B p65 in Human Airway Epithelial Cells
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Lee Hyun-Jae
Lee Choong-Jae
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Abstract
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In the present study, we investigated whether oleanolic acid, an anti-inflammatory natural product, significantlyaffects the phorbol 12-myristate 13-acetate (PMA)-induced gene expression of airway MUC5AC mucin. Confluent NCIH292cells were pretreated with oleanolic acid for 30 min and then stimulated with PMA for 24 h or the indicated periods.
The MUC5AC mucin mRNA expression was measured by RT-PCR. Production of MUC5AC mucin protein was measuredby ELISA. To elucidate the action mechanism of oleanolic acid, effect of oleanolic acid on PMA-induced NF-?B signalingpathway was investigated by western blot analysis. The results were as follows: (1) Oleanolic acid significantly inhibitedthe production of MUC5AC mucin protein and down-regulated the expression of MUC5AC mucin mRNA; (2) Oleanolicacid inhibited PMA-induced activation (phosphorylation) of inhibitory kappa B kinase (IKK), and thus phosphorylation anddegradation of inhibitory kappa Ba (I?B?); (3) Oleanolic acid inhibited PMA-induced nuclear translocation of nuclearfactor kappa B (NF-?B) p65, led to the down-regulation of MUC5AC mucin gene expression in NCI-H292 cells. Theseresults suggest that oleanolic acid can regulate the gene expression of mucin by acting on airway epithelial cells throughregulation of NF-?B signaling pathway.
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KEYWORD
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Pulmonary, Oleanolic acid, Mucin
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